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Table 1 The values of the transcripts retained in samples reverse transcribed on Day 1 and Day 8 post treatment

From: Application of NeuroTrace staining in the fresh frozen brain samples to laser microdissection combined with quantitative RT-PCR analysis

 

Day 1

Day 8

Untreated

EtOH/NT

Untreated

EtOH/NT

18S rRNA

100 ± 7

37 ± 11***

100 ± 5

49 ± 10***

GAPDH

100 ± 9

32 ± 9***

100 ± 9

32 ± 8***

β-actin

100 ± 13

26 ± 8***

100 ± 12

29 ± 9***

Map2

100 ± 9

43 ± 10**

100 ± 11

45 ± 11***

cFos

100 ± 15

57 ± 13*

100 ± 11

56 ± 13*

Arc

100 ± 11

37 ± 9**

100 ± 11

52 ± 14*

BDNF

100 ± 6

34 ± 8***

100 ± 12

38 ± 10**

TrkB

100 ± 12

35 ± 11**

100 ± 6

50 ± 11**

  1. p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t-test between untreated samples and ethanol-fixed, NeuroTrace-stained samples (EtOH/NT) samples. There were no significant differences between the transcript retained values (%) of the samples reverse transcribed on Day 1 and Day 8 after the treatment, for any of the quantified genes