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Table 1 Exon-trap-based HPRT-gene targeting in HT1080 cells

From: Construction and applications of exon-trapping gene-targeting vectors with a novel strategy for negative selection

Experiment Number of cells transfected Total number of puromycin-resistant colonies Gene-targeting efficiency
1 4.0 × 107 411 0.8% (2/244)
2 4.0 × 107 614 0.4% (2/501)
  1. The targeting vector pHPRT-SH 2A-EGFP-2A-Puro was I-SceI linearized and transfected into HT1080 cells, and the gene-targeting efficiency was determined as described in “Methods”.