Experiment
|
Number of cells transfected
|
Total number of puromycin-resistant colonies
|
Gene-targeting efficiency
|
---|
1
|
4.0 × 107
|
411
|
0.8% (2/244)
|
2
|
4.0 × 107
|
614
|
0.4% (2/501)
|
- The targeting vector pHPRT-SH 2A-EGFP-2A-Puro was I-SceI linearized and transfected into HT1080 cells, and the gene-targeting efficiency was determined as described in “Methods”.