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Table 1 Exon-trap-based HPRT-gene targeting in HT1080 cells

From: Construction and applications of exon-trapping gene-targeting vectors with a novel strategy for negative selection

Experiment

Number of cells transfected

Total number of puromycin-resistant colonies

Gene-targeting efficiency

1

4.0 × 107

411

0.8% (2/244)

2

4.0 × 107

614

0.4% (2/501)

  1. The targeting vector pHPRT-SH 2A-EGFP-2A-Puro was I-SceI linearized and transfected into HT1080 cells, and the gene-targeting efficiency was determined as described in “Methods”.
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BMC Research Notes

ISSN: 1756-0500

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