|
Experiment
|
DT-A
|
Random integration frequencya
|
Fold decrease
|
|---|
|
1
|
−
|
1.0 × 10−4 (1)
|
25
|
|
+
|
4.1 × 10−6 (0.04)
|
|
2
|
−
|
2.8 × 10−5 (1)
|
8.5
|
|
+
|
3.3 × 10−6 (0.12)
|
|
3
|
−
|
6.2 × 10−5 (1)
|
5.6
|
|
+
|
1.1 × 10−5 (0.18)
|
- The targeting vector pHPRT-SH 2A-EGFP-2A-Puro was I-SceI linearized and transfected into HT1080 cells by using the Nucleofector II system (Experiments 1 and 2) or the MaxCyte devise (Experiment 3). DT-A denotes the presence (+) or absence (−) of the ExTraPANS cassette (SA–IRES–DT-A–polyA sequence) in the targeting vector. The random-integration frequency was determined as described in “Methods”.
-
aNumbers in parentheses represent relative random-integration frequencies.
