Skip to main content

Advertisement

Table 3 Effect of the novel negative selection cassette on targeting efficiency

From: Construction and applications of exon-trapping gene-targeting vectors with a novel strategy for negative selection

Experiment Selection marker DT-A Gene-targeting efficiency Fold increase
1 2A-EGFP-2A-Puro 0.4% (1/250) 9.0
+ 3.6% (2/55)
2 2A-Puro 1.7% (2/116) 2.8
+ 4.7% (2/43)
3 IRES2-Hyg 0% (0/133) >4.6
+ 3.7% (1/27)
4 IRES-Puro 0% (0/7) >3.5
+ 50% (1/2)
  1. Human HT1080 cells and mouse ES cells were used for targeted disruption of the HPRT locus (Experiments 1–3) or the Rosa26 locus (Experiment 4), respectively. DT-A denotes the presence (+) or absence (−) of the ExTraPANS cassette (SA–IRES–DT-A–polyA sequence) in the targeting vector.