Skip to main content

Table 3 Effect of the novel negative selection cassette on targeting efficiency

From: Construction and applications of exon-trapping gene-targeting vectors with a novel strategy for negative selection

Experiment

Selection marker

DT-A

Gene-targeting efficiency

Fold increase

1

2A-EGFP-2A-Puro

−

0.4% (1/250)

9.0

+

3.6% (2/55)

2

2A-Puro

−

1.7% (2/116)

2.8

+

4.7% (2/43)

3

IRES2-Hyg

−

0% (0/133)

>4.6

+

3.7% (1/27)

4

IRES-Puro

−

0% (0/7)

>3.5

+

50% (1/2)

  1. Human HT1080 cells and mouse ES cells were used for targeted disruption of the HPRT locus (Experiments 1–3) or the Rosa26 locus (Experiment 4), respectively. DT-A denotes the presence (+) or absence (−) of the ExTraPANS cassette (SA–IRES–DT-A–polyA sequence) in the targeting vector.