Fig. 1From: Further characterisation of transmissible spongiform encephalopathy phenotypes after inoculation of cattle with two temporally separated sources of sheep scrapie from Great BritainDiscriminatory Western immunoblot of brain samples from cattle inoculated with the pre-1975 and post-1990 scrapie brain pools. Lanes 1–9 cattle inoculated intracerebrally with the pre-1975 scrapie pool: P75-1, P75-2, P75-3, P75-4, P75-5, P75-6, P75-7, P75-8 and P-75-9. Lanes 10–16 cattle inoculated intracerebrally with the post-1990 scrapie pool: P90-2, P90-3, P90-1, P90-4, P90-5, P90-6 and P90-7. Lanes L, H, C, O controls: L-type BSE, H-type BSE, classical BSE, ovine scrapie. Lanes M molecular mass marker. Animal P90-4, sample lane 13, was an outlier with a lower molecular mass of the unglycosylated band with mAbs Sha31 and SAF84 compared to the other samples previously tested with mAb 6H4. The sample of the other outlier P90-5 (determined previously by testing caudal medulla), sample lane 14, consisted here of rostral medulla and gave a molecular profile similar to the others of the group as observed in the original blot when both brain samples were tested [2]. The lane numbers of those cattle that provided the inocula for bank voles and mice are underlined. Back to article page