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Table 1 Animal details and outcome of cattle inoculated intracerebrally with two scrapie pools

From: Further characterisation of transmissible spongiform encephalopathy phenotypes after inoculation of cattle with two temporally separated sources of sheep scrapie from Great Britain

Case ORF 23 bp 12 bp Death (mpi) Reason for cull (clinical and/or pathological diagnosis) TSE test result
Pre-1975 pool
 P75-1 6:6 N192 het −/− −/− 18 TSE suspect (dull syndrome) Positive
 P75-2 6:6 Q78 het +/− +/− 21 TSE suspect (dull syndrome) Positive
 P75-3 6:6 WT −/− −/− 24 TSE suspect (dull syndrome) Positive
 P75-4 6:6 N192 het −/− −/− 24 TSE suspect (dull syndrome) Positive
 P75-5 6:5 Q78 het +/− +/+ 24 TSE suspect (dull syndrome) Positive
 P75-6 6:6 Q78 het +/− +/− 26 TSE suspect (dull syndrome) Positive
 P75-7 6:6 WT −/− −/− 29 TSE suspect (dull syndrome) Positive
 P75-8 6:6 WT −/− −/− 34 TSE suspect (dull syndrome) Positive
 P75-9 6:6 Q78 het +/− +/− 56 TSE suspect (dull syndrome) Positive
 P75-10 6:6 Q78 het +/− +/− 91 Muscle trauma Negative
Post-1990 pool
 P90-1 6:6 N192 het −/− −/− 18 TSE suspect (dull syndrome) Positive
 P90-2 6:5 Q78 het +/− +/+ 24 TSE suspect (nervous syndrome) Positive
 P90-3 6:6 Q78 het +/− +/− 25 TSE suspect (dull syndrome) Positive
 P90-4 6:5 Q78 het +/− +/+ 30 TSE suspect (dull syndrome) Positive
 P90-5 6:6 Q78 hom +/+ +/+ 32 TSE suspect (nervous syndrome) Positive
 P90-6 6:6 Q78 het +/− +/− 35 TSE suspect (dull syndrome) Positive
 P90-7 6:6 WT −/− −/− 54 TSE suspect (dull syndrome) Positive
 P90-8 6:6 Q78 het +/− +/− 84 TSE suspect (difficulty rising, dullness) Negative
 P90-9 6:6 Q78 het +/− +/− 99 Difficulty rising, stiffness, hypophosphataemia Negative
 P90-10 6:6 Q78 het +/− +/− 120 End of study Negative
Saline solution
 CSa-1 6:6 WT −/− −/− 60 Difficult to handle (cryptorchid) Negative
 CSa-2 6:6 Q78 hom +/− +/− 101 Stiffness, visual impairment (strabismus, exophthalmos) Negative
 CSa-3 6:6 Q78 het +/− +/− 103 Osteoarthrosis Negative
 CSa-4 6:5 WT −/− +/− 115 Vertebral fracture Negative
 CSa-5 6:6 Q78 het N192 het +/− +/− 120 End of study Negative
Scrapie-free brain
 CB-1 6:6 Q78 het +/− +/− 82 Spastic syndrome, osteoarthrosis Negative
 CB-2 6:6 WT −/− −/− 82 Spastic syndrome, osteoarthrosis Negative
 CB-3 6:6 Q78 het +/− +/− 120 End of study Negative
 CB-4 6:6 Q78 het N192 het +/− +/− 120 End of study Negative
 CB-5 6:6 WT −/− −/− 120 End of study Negative
  1. ORF open reading frame of the bovine PrP gene detailing the number of N-terminal octapeptide repeats, the silent polymorphisms Q78 and N192, either homozygous (hom) or heterozygous (het) at position 78 and 192 of the ORF respectively compared to the wild type (WT); and the 23 and 12 bp indels ( deletion allele, + insertion allele) of the promoter PrP gene, mpi months post inoculation, rounded down to the nearest month.