Further characterisation of transmissible spongiform encephalopathy phenotypes after inoculation of cattle with two temporally separated sources of sheep scrapie from Great Britain

Konold, Timm; Nonno, Romolo; Spiropoulos, John; Chaplin, Melanie J; Stack, Michael J; Hawkins, Steve A C; Cawthraw, Saira; Wilesmith, John W; Wells, Gerald A H; Agrimi, Umberto; Di Bari, Michele A; Andréoletti, Olivier; Espinosa, Juan C; Aguilar-Calvo, Patricia; Torres, Juan M

doi:10.1186/s13104-015-1260-3

Table 3 Mean survival times and attack rate in transgenic mice inoculated with two ovine scrapie pools, two single case sources of bovine scrapie and single case sources of classical and atypical BSE on primary and second passage

From: Further characterisation of transmissible spongiform encephalopathy phenotypes after inoculation of cattle with two temporally separated sources of sheep scrapie from Great Britain

Inocula	Post-1990 scrapie pool	P90-1	Pre-1975 scrapie pool	P75-7	Bovine C-type BSE	Ovine C-type BSE	H-type BSE	L-type BSE
BoPrP-tg110
1st passage	643 (1/5) 19 + 21K	173 ± 3 (6/6) 21K	457 ± 60 (3/6) 19K	203 ± 5 (6/6) 19K	295 ± 12 (6/6) BSE like^a	234 ± 5 (6/6) BSE like	292 ± 5 (6/6) H-type^b	207 ± 7 (6/6) L-type
2nd passage	282 ± 6 (6/6) 19K	190 ± 16 (6/6) 21K	191 ± 4 (6/6) 19K	200 ± 9 (6/6) 19K	265 ± 35 (6/6) BSE like	234 ± 3 (6/6) BSE like	296 ± 7 (6/6) H-type	199 ± 1 (6/6) L-type
PoPrP-tg001
1st passage	>650 (0/6)	>650 (0/6)	>650 (0/6)	>650 (0/6)	498 ± 9^c (2/12)	458 ± 11^c (15/15)	>650 (0/6)^b	>650 (0/6)
2nd passage	>650 (0/6)	>650 (0/6)	>650 (0/6)	>650 (0/6)	198 ± 6^c (15/15)	162 ± 4^c (13/13)	>650 (0/6)^b	>650 (0/6)
MuPrP-tga20
1st passage	571 ± 31 (3/6) 21K	440 ± 3 (5/6)	441 ± 67 (6/6) 21K	480 ± 13 (6/6)	473 ± 24 (6/6) BSE like	450 ± 48 (6/6) BSE like	ND	ND
2nd passage	159 ± 2 (6/6) 21K	408 ± 33 (3/6)	146 ± 41 (3/3) 21K	ND	147 ± 3 (6/6) BSE like	117 ± 3 (6/6) BSE like	ND	ND
HuPrP-tg340
1st passage	ND	>650 (0/5)	ND	>650 (0/6)	>700 (1/12) BSE like	615 ± 84 (4/6) BSE like^d	>700 (0/6)^b	629 ± 35 (5/5) L-type
2nd passage	ND	>650 (0/5)	ND	>650 (0/6)	690 ± 35 (5/6) BSE like	564 ± 39 (5/5) BSE like	>700 (0/6)^b	684 ± 45 (4/4) L-type
OvPrP-tg338
1st passage	480 ± 19 (6/6) 21K	>638 (0/6)	69 ± 1 (6/6) 21K	148 ± 2 (5/6) 19K	704 ± 36 (6/7) BSE like^f	560 ± 60 (5/5) BSE-like^f	595 ± 18 (8/8) H-type^g	432 ± 19 (6/6) BSE-like^f
2nd passage	ND	545 (1/5)^e 19K	ND	ND	ND	178 ± 2 (4/4) BSE-like^f	319 ± 10 (6/6) H-type^g	141 ± 2 (7/7) BSE-like^f

Survival times are displayed in days with standard error of the mean. K denotes the molecular masses (in kDa) of the unglycosylated PrP^res band determined by WB.
ND not done.
^aInoculum from clinical BSE suspect, 8 year-old Holstein-Friesian cow, UK.
^bInoculum from 03-2095, clinically healthy ≥8 year-old cow, France [9, 44].
^cPreviously published data from UK and French cases: C-type BSE (BSE2, 8 year-old Hereford crossbred cow, clinical suspect), ovine BSE (pool from 7 ARQ/ARQ sheep intracerebrally infected with brainstem from a naturally affected BSE cow in France) [31].
^dBrain pool of ARQ/ARQ sheep inoculated with brain from a naturally infected BSE cow [26].
^eOne additional inoculated mouse died at 535 days but no TSE diagnosis was possible.
^fPreviously published data from French cases: Ovine BSE (case ARQ1), C-type BSE (case 3), L-type BSE (case 7) [29].
^gPreviously published data from French case 2 [30].

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ISSN: 1756-0500

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