Skip to main content

Table 3 Mean survival times and attack rate in transgenic mice inoculated with two ovine scrapie pools, two single case sources of bovine scrapie and single case sources of classical and atypical BSE on primary and second passage

From: Further characterisation of transmissible spongiform encephalopathy phenotypes after inoculation of cattle with two temporally separated sources of sheep scrapie from Great Britain

Inocula Post-1990 scrapie pool P90-1 Pre-1975 scrapie pool P75-7 Bovine C-type BSE Ovine C-type BSE H-type BSE L-type BSE
BoPrP-tg110
 1st passage 643 (1/5) 19 + 21K 173 ± 3 (6/6)
21K
457 ± 60 (3/6) 19K 203 ± 5 (6/6)
19K
295 ± 12 (6/6) BSE likea 234 ± 5 (6/6) BSE like 292 ± 5 (6/6) H-typeb 207 ± 7 (6/6) L-type
 2nd passage 282 ± 6 (6/6)
19K
190 ± 16 (6/6)
21K
191 ± 4 (6/6)
19K
200 ± 9 (6/6)
19K
265 ± 35 (6/6) BSE like 234 ± 3 (6/6) BSE like 296 ± 7 (6/6) H-type 199 ± 1 (6/6) L-type
PoPrP-tg001
 1st passage >650 (0/6) >650 (0/6) >650 (0/6) >650 (0/6) 498 ± 9c (2/12) 458 ± 11c (15/15) >650 (0/6)b >650 (0/6)
 2nd passage >650 (0/6) >650 (0/6) >650 (0/6) >650 (0/6) 198 ± 6c (15/15) 162 ± 4c (13/13) >650 (0/6)b >650 (0/6)
MuPrP-tga20
 1st passage 571 ± 31 (3/6)
21K
440 ± 3 (5/6) 441 ± 67 (6/6)
21K
480 ± 13 (6/6) 473 ± 24 (6/6) BSE like 450 ± 48 (6/6) BSE like ND ND
 2nd passage 159 ± 2 (6/6)
21K
408 ± 33 (3/6) 146 ± 41 (3/3)
21K
ND 147 ± 3 (6/6) BSE like 117 ± 3 (6/6) BSE like ND ND
HuPrP-tg340
 1st passage ND >650 (0/5) ND >650 (0/6) >700 (1/12) BSE like 615 ± 84 (4/6) BSE liked >700 (0/6)b 629 ± 35 (5/5) L-type
 2nd passage ND >650 (0/5) ND >650 (0/6) 690 ± 35 (5/6) BSE like 564 ± 39 (5/5) BSE like >700 (0/6)b 684 ± 45 (4/4) L-type
OvPrP-tg338
 1st passage 480 ± 19 (6/6)
21K
>638 (0/6) 69 ± 1 (6/6)
21K
148 ± 2 (5/6)
19K
704 ± 36 (6/7) BSE likef 560 ± 60 (5/5) BSE-likef 595 ± 18 (8/8) H-typeg 432 ± 19 (6/6) BSE-likef
 2nd passage ND 545 (1/5)e 19K ND ND ND 178 ± 2 (4/4) BSE-likef 319 ± 10 (6/6) H-typeg 141 ± 2 (7/7) BSE-likef
  1. Survival times are displayed in days with standard error of the mean. K denotes the molecular masses (in kDa) of the unglycosylated PrPres band determined by WB.
  2. ND not done.
  3. aInoculum from clinical BSE suspect, 8 year-old Holstein-Friesian cow, UK.
  4. bInoculum from 03-2095, clinically healthy ≥8 year-old cow, France [9, 44].
  5. cPreviously published data from UK and French cases: C-type BSE (BSE2, 8 year-old Hereford crossbred cow, clinical suspect), ovine BSE (pool from 7 ARQ/ARQ sheep intracerebrally infected with brainstem from a naturally affected BSE cow in France) [31].
  6. dBrain pool of ARQ/ARQ sheep inoculated with brain from a naturally infected BSE cow [26].
  7. eOne additional inoculated mouse died at 535 days but no TSE diagnosis was possible.
  8. fPreviously published data from French cases: Ovine BSE (case ARQ1), C-type BSE (case 3), L-type BSE (case 7) [29].
  9. gPreviously published data from French case 2 [30].