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Table 3 Method validation of chemical markers in R. purpurea tubers methanol extract through HPTLC

From: Nutritional evaluation, antioxidant studies and quantification of poly phenolics, in Roscoea purpurea tubers

Parameters Protocatechuic acid Vanillic acid Syringic acid Kaempferol Ferullic acid
Linearity range (ng) 100–600 100–600 100–600 100–600 100–600
Regression coefficient (r2) 0.993 0.999 0.999 0.999 0.992
LOD (ng) 35 35 35 35 35
LOQ (ng) 100 100 100 100 100
Rf 0.48 0.60 0.53 0.59 0.58
Wavelength (nm) 305 305 285 305 305
  1. (Values are mean, n = 3).
  2. ng nanogram.