Alignment of PanM proteins used in ConSuf analyses. Surface exposed residues with high conservation highlighted in black. These residues form the predicted PanD binding site. The asterisk indicates identifies residue Asn45, which was found to be required for PanM binding to pro-PanD . The bar atop the sequence on the lower panel spans residues Leu66-Gly76, which was also identified by structural studies to be required for PanZ/PanM:PanD interactions .