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Fig. 1 | BMC Research Notes

Fig. 1

From: Insulin and IGF-1, but not 17β-estradiol, alter the subcellular localization of MIER1α in MCF7 breast carcinoma cells

Fig. 1

Insulin treatment reduces nuclear localization of MIER1α. MCF7 cells were transfected with myc-tagged mier1α and treated 24 h later with either vehicle (panels ac) or 10 ug/ml insulin (panels df). Cells were fixed 4 h after the addition of insulin and localization was analyzed by confocal microscopy. Random fields were selected and the staining pattern of each cell within the field was scored as ‘nuclear’, ‘whole cell’ or ‘cytoplasmic’, using sequential Z-stage scanning. A Shown are Z-stacks compiled into individual images, illustrating stained nuclei (DAPI; panels a, d), MIER1α localization (9E10 anti-myc tag antibody and an AlexaFluor-488 secondary antibody; panels b, e) and merged channels (panels c, f); arrowheads show examples of nuclear staining and arrows show whole cell staining. B Histogram showing the results of 3 independent experiments; the MIER1α localization pattern of >1000 cells was scored. Plotted is the percentage of cells in each category ±SD; Asterisk indicates that the difference was statistically significant, p < 0.05 (Fisher’s exact test)

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