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Fig. 3 | BMC Research Notes

Fig. 3

From: Simple viral/minimal piggyBac hybrid vectors for stable production of self-inactivating gamma-retroviruses

Fig. 3

a Percentage of ZsGreen, turboRFP or ZsGreen/turboRFP positive packaging cells 21 days after transfection (i.e. stably positive) with tested plasmids. RV wild type retroviral vector A, RV(SIN) SIN retroviral vector B, RV(SIN)minPB SIN retroviral/minimal piggyBac hybrid vector C, Control negative control vector D. b, c The viral titers (IU infectious units/ml) in the medium of two sequential passages of retrovirus originated from stably integrated constructs. Twenty-one days after transfection with the three retroviral plasmids, only virus-producing cells expressing ZsGreen (populations A, B and C’) or both ZsGreen and turboRFP (population C) were purified and their conditioned medium applied to other packaging cells for infection (1st infection cycle, b). Only cells expressing the viral marker (ZsGreen) after the 1st infection cycle were purified and their conditioned medium tested for retroviral SIN function using new target cells (2nd infection cycle, c). (Data are expressed as mean ± SE; n = 4 experiments; *P < 0.05)

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