Fig. 5

Growth retardation of S. cerevisiae SUBSC01 at 32.5 and 45 °C with different dextrose concentrations. Cell culturability was assessed through OD₆₀₀ and the formation of CFUs at 32.5 °C (a, b), and at 45 °C (c, d) with different dextrose concentrations, i.e., 0.04 g/l (1X), 0.12 g/l (3X), 0.2 g/l (5X), 0.28 g/l (7X), 0.36 g/l (9X). Cells were grown as stated in “Methods”, and aliquots were removed at the specific time intervals of 90, 180, 270, 360 and 450 min. A complete elimination of culturable cells were observed at 45 °C with 7X and 9X dextrose concentrations (c, d)