Fig. 2
From: ABCG2 impairs the activity of the aurora kinase inhibitor tozasertib but not of alisertib
ABCG2 depletion using a second lentiviral vector (LeGO-X-GFP2) with dsRedExpress as marker encoding an shRNA targeting the bicistronic ABCG2-IRES-eGFP mRNA of the first vector (LeGO-iG2-ABCG2) thereby depleting eGFP and ABCG2 expression. a Fluorescence pictures indicating dsRedExpress (encoded as marker by LeGO-X-GFP2) and eGFP fluorescence in UKF-NB-3 cells transduced with LeGO-iG2-ABCG2 (UKF-NB-3ABCG2) or LeGO-iG2-ABCG2 and LeGO-X-GFP2 (UKF-NB-3ABCG2-XGFP2). b ABCG2 levels in UKF-NB-3 cells, UKF-NB-3 cells transduced with the empty LeGO-iG2 vector (UKF-NB-3iG2), UKF-NB-3ABCG2 cells, or UKF-NB-3ABCG2-XGFP2 cells as determined by flow cytometry and expressed as relative fluorescence units (rfu). *P < 0.05 relative to UKF-NB-3