Fig. 3
From: ABCG2 impairs the activity of the aurora kinase inhibitor tozasertib but not of alisertib
Effects of ABCG2 depletion on UKF-NB-3ABCG2 cell sensitivity to tozasertib and the cytotoxic ABCG2 substrate mitoxantrone. Concentrations that reduce cell viability by 50 % after 120 h incubation (IC50) were determined by MTT assay in UKF-NB-3 cells, UKF-NB-3 cells transduced with a control vector (UKF-NB-3iG2), UKF-NB-3 cells transduced with the lentiviral vector LeGO-iG2-ABCG2 encoding for ABCG2 (UKF-NB-3ABCG2), and UKF-NB-3ABCG2 cells in which ABCG2 was depleted using a lentiviral vector encoding an shRNA directed against the mRNA of eGFP and ABCG2 (LeGO-X-GFP2) of the LeGO-iG2-ABCG2 vector (UKF-NB-3ABCG2-XGFP2). *P < 0.05 relative to UKF-NB-3