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Table 2 Schematic experimental design of the two conducted experiments in which two clearly distinct activities are described

From: Effects of freezing storage on the DNA extraction and microbial evaluation from anaerobic digested sludges

Aim

Samples characteristics

Sample origin

Schedule of DNA extraction

Comparing the extracted DNA concentration in fresh and frozen samples

4 types of samples named A, B, C and D

Sludge from anaerobic digestion of WWTP

For each sample: DNA extraction from fresh sample and DNA extraction from 10-days frozen sample

Verifying the preservation of the extracted DNA concentration in frozen samples

2 samples, named E and F

Sludge from anaerobic digestion of OFMSW

Repeated DNA extractions from aliquots of the same sample after different storage periods