Analysis of the leakage of gene repression by an artificial TetR-regulated promoter in cyanobacteria

BMC Research Notes

Table 1 Promoter data from previous in vivo measurements [8]

Promoter	Point mutation^a	Strength^b		Induction^c
Promoter	Point mutation^a	Induced	Repressed	Induction^c
L09	C	15.6 ± 0.1	2.88 ± 0.01	5.15 ± 0.01
L10	T	17.6 ± 0.1	0.235 ± 0.003	71 ± 1
L11	G	19.1 ± 0.1	0.236 ± 0.003	77 ± 1
L12	A	0.043 ± 0.003	0.022 ± 0.003	1.9 ± 0.3

^aThe location of the point mutation in a L promoter is shown in Fig. 1
^bThe promoter strength in the induced and repressed conditions is measured by flow cytometer to detect EYFP emission in a single cell from liquid culture of cyanobacterium Synechocystis PCC 6803 treated with and without 100 ng mL⁻¹ aTc, respectively. The unit-less promoter strength is normalized to the reference rnpB promoter’s strength in the respective condition
^cThe induction is the ratio of induced to repressed promoter strength (±SD)

ISSN: 1756-0500