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Table 1 Most common problems reported or observed during on-site evaluation visits of the eight selected health institution medical laboratories from December 2011 to December 2012

From: External quality assessment for acid fast bacilli smear microscopy in eastern part of Ethiopia

Problems found

Laboratories N (%)

Laboratory safety

 No separate area for TB work

8 (100)

 No adequate ventilation

5 (62.5)

 No biohazard waste bin with a lid

4 (50)

 Improper use of PPE

7 (87.5)

 Inappropriate cleaning procedure

7 (87.5)

Laboratory reagents

 Expired reagents or no label

3 (37.5)

 Not filtered before use

7 (87.5)

 Filtered once a month

4 (50)

Laboratory supplies

 No staining rack

5 (62.5)

 No wire loop

6 (75)

 No funnel

4 (50)

 No analytical balance

8 (100)

Microscope

 Not sufficient

4 (50)

 Inadequate light source

6 (75)

 Objective not cleaned after positive slide

7 (87.5)

 No routine care/daily maintenance

7 (87.5)

Smearing and staining procedures

 Background staining not acceptable

3 (37.5)

 Background material doesn’t represent sputum

4 (50)

 Inappropriate smear thickness

5 (62.5)

 Inappropriate smear size

4 (50)

 Report with grading

6 (75)

 Include control smears

  Daily

2 (25)

  New batch of reagents

5 (62.5)

  Never

2 (25)

Time taken to examine 100 fields

 5 min

4 (50)

 10 min

3 (37.5)

 15 min

1 (12.5)

Administrative

 High workload

4 (50)

 Incomplete laboratory request form

7 (87.5)

 No NTP approved report forms

3 (37.5)

EQA

 Participated

7 (87.5)

 Rechecking result received

3 (37.5)