Fig. 6From: Genome-wide analysis of overlapping genes regulated by iron deficiency and phosphate starvation reveals new interactions in Arabidopsis rootsReverse transcription–quantitative PCR (RT–qPCR) detected expression of Pi- and Fe-responsive marker genes under Pi deficiency (a) or Fe deficiency or Pi deficiency without or with low Fe concentrations (b). Total RNA was isolated from roots in wild type or phr1 mutant plants and qPCR was performed. Expression levels are relative to normal controls. Error bars represent SD of biological replicates from three independent experiments. Data significantly different from the corresponding controls are indicated (‘−Pi’ versus ‘+Pi’, *P < 0.05, **P < 0.01; ‘−Fe’ versus ‘−Fe−Pi’, +P < 0.05, ++P < 0.01; ‘−Pi’ versus ‘5 µMFe−Pi’, ′P < 0.05, ″P < 0.01; Student’s t test)Back to article page