Fig. 1

Images of orangutan iPSCs and derivatives. Data pertaining to KB10973 iPSC colony 1 (Panels a–k) and KB10460 iPSC colony 1 (panels l–v) are provided, as described in “Methods” Section. Light microscope images of iPSCs are shown in panels (a, l). Alkaline phosphatase staining is depicted in panels (b, m). Images of iPSCs immunostained for TRA-1-60 (panels c, n), TRA-1-81 (panels d, o), SSEA4 (panels e, p), OCT4 (panels f, q), SOX2 (panels g, r), and NANOG (panels h, s) are shown. DAPI nuclear counterstaining is not shown for the purpose of image clarity. Panels t–v provide the results of in vitro differentiation assays conducted on KB10973 iPSC colony 1 (panels i–k) and KB10460 iPSC colony 1. Cell populations derived from each of the three germ layers were detected by immunostaining for AFP (endoderm; panels i, t), SMA (mesoderm; panels j, u), and beta-III-tubulin (ectoderm; panels k, v). Nuclei were counterstained with DAPI (blue) in panels t–v