TY - JOUR AU - Pineda, Gabriel AU - Shen, Zhouxin AU - de Albuquerque, Claudio Ponte AU - Reynoso, Eduardo AU - Chen, Jeffrey AU - Tu, Chi-Chiang AU - Tang, Wingchung AU - Briggs, Steve AU - Zhou, Huilin AU - Wang, Jean Y. J. PY - 2015 DA - 2015/10/29 TI - Proteomics studies of the interactome of RNA polymerase II C-terminal repeated domain JO - BMC Research Notes SP - 616 VL - 8 IS - 1 AB - Eukaryotic RNA polymerase II contains a C-terminal repeated domain (CTD) consisting of 52 consensus heptad repeats of Y1S2P3T4S5P6S7 that mediate interactions with many cellular proteins to regulate transcription elongation, RNA processing and chromatin structure. A number of CTD-binding proteins have been identified and the crystal structures of several protein-CTD complexes have demonstrated considerable conformational flexibility of the heptad repeats in those interactions. Furthermore, phosphorylation of the CTD at tyrosine, serine and threonine residues can regulate the CTD-protein interactions. Although the interactions of CTD with specific proteins have been elucidated at the atomic level, the capacity and specificity of the CTD-interactome in mammalian cells is not yet determined. SN - 1756-0500 UR - https://doi.org/10.1186/s13104-015-1569-y DO - 10.1186/s13104-015-1569-y ID - Pineda2015 ER -