Fig. 1
From: Proteomics studies of the interactome of RNA polymerase II C-terminal repeated domain
Proteomic analysis of proteins pulled-down by phosphorylated CTD peptides. a Summary of experimental strategy. Six CTD-peptides with phosphorylation sites marked in red and biotinylation at the N-terminus marked as a circle were synthesized and used as affinity probes to pull-down proteins from HeLa nuclear extracts. b A representative silver stained gel of proteins pulled-down by the six CTD-peptides. c, d Graphical representations of bioinformatics analysis of CTD-interacting proteins separated by GO terms in biological process (c) or molecular function (d). The CTD-interaction proteins are listed in Table 1