Table 1 Microsatellite loci for Jaera albifrons and multiplex PCR conditions
From: Microsatellite markers for the Jaera albifrons species complex (marine isopods)
Locus | F primer seq. (5′ to 3′) | R primer seq. (5′ to 3′) | Motif | Range size (pb) | GenBank Accession no. | PCR multiplex | Dye | Primer (10 µM) | Init. temp. | Fin. temp. | No. final cycles | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
F** (µl) | F (µl) | R (µl) | |||||||||||
Ja30 | CGTCATTTATGCGTGCGGTA | CGTCCATTCTTCATCTGACTGC | (TC)9 | 173–185 | KP749881 | 1 | PET | 0.1 | 0.2 | 0.3 | 60 | 50 | 20 |
Ja37 | TCGAGCATCATACGACGACA | GCCTATAGTGTCTTTGGCACC | (GA)8 | 101–115 | KP749883 | FAM | 0.4 | 0 | 0.4 | ||||
Ja41 | GGTGTTGGCGGAAAGATTCT | AGCCTACTCTACCGTCTGTT | (GA)8 | 172–180 | KP749885 | NED | 0.1 | 0.2 | 0.3 | ||||
Ja91 | ATCGGCATTCCACTGAGAGG | CCAGTCTTGAATGCCTTGGG | (CCT)6 | 073–082 | KP749896 | VIC | 0.1 | 0.2 | 0.3 | ||||
Ja01 | CAATCTTCGTCTTGGCGTCT | CACTGTGGGCTTAGGATTGT | (AG)8 | 226–247 | KP749875 | 2 | FAM | 0.5 | 0 | 0.5 | 57 | 47 | 20 |
Ja02 | TGCCACCAATTAACCAAACA | CGACAGCCATAATGATCACC | (CT)10N16(CT)5 | 182-212 | KP749876 | NED | 0.1 | 0.2 | 0.3 | ||||
Ja35 | TGGATTCACATTGCTTCCTGG | TCACGCATTCTAGTCTACGCT | (AT)8 | 173–179 | KP749882 | VIC | 0.3 | 0.1 | 0.4 | ||||
Ja47 | GGTGCTTAATGTAGTAGAGCGG | ATGGAACCACAAAACGGACG | (TG)10 | 116–124 | KP749886 | 3 | NED | 0.1 | 0.2 | 0.3 | 60 | 50 | 20 |
Ja56 | ACTAACACAAACATGCACTTACA | ATTGTTAGGTGCCTGCCATT | (ACA)6 | 261–276 | KP749888 | FAM | 0.3 | 0.3 | 0.6 | ||||
Ja58 | CCCACTGGACCACTACTTGA | ACCCTATCGTTGTAGTTGAGGT | (CT)5 | 186–188 | KP749889 | VIC | 0.3 | 0.1 | 0.4 | ||||
Ja99 | ATCAAACATGCAGCGGTGTC | AGGCATCTCAGAGGAATCACTT | (TC)5 | 152–154 | KP749898 | PET | 0.1 | 0.2 | 0.3 | ||||
Ja21 | TTCAAATTAAATGCAACGATG | CATTATTCTGAAGCTGTTGGTC | (GA)10 | 272–286 | KP749878 | 4 | NED | 0.25 | 0 | 0.25 | 60 | 50 | 25 |
Ja71 | CTGCCCTATCAGTTGAGCTT | CACAGTGCCATCAACAAAGC | (GA)7 | 263–269 | KP749892 | VIC | 0.3 | 0 | 0.3 | ||||
Ja78 | CACTCTACAGCAGCATAAGAGT | AGAACAAGGCAAGATGAGCTC | (AC)8 | 243–254 | KP749893 | PET | 0.2 | 0.1 | 0.3 | ||||
Ja94 | TGGTCCGATCGTGAGTTTCA | CCTTCGGATGGTAATAGGGCA | (AT)6 | 101–111 | KP749897 | PET | 0.1 | 0.2 | 0.3 | ||||
Ja22 | GCAAACTCGATCACCATTGGA | ACCCACCGTCGTCTATCAAC | (CT)9 | 106–128 | KP749879 | 5 | VIC | 0.2 | 0.1 | 0.3 | 60 | 50 | 23 |
Ja64 | TAAGCCGAGTCTCAACAGCA | CCCGTGCATAGCGAACAG | (TG)5N22(TG)5 | 119–127 | KP749890 | PET | 0.1 | 0.2 | 0.3 | ||||
Ja66 | CGCAAGTACAAATTTCTCTATGCT | TCGAACGGTACTCAATGTGAAG | (TAGA)5 | 175–187 | KP749891 | FAM | 0.4 | 0 | 0.4 | ||||
Ja80 | TGATGGATGAGAGGTGATCGT | TTGATTCAGATCCGATACCATGT | (GA)6 | 154–154 | KP749894 | NED | 0.2 | 0.1 | 0.3 | ||||
Ja13 | ATCCTTCATGAGTCCCGAGT | AAGTATGTCCGAACTACCGC | (GA)12 | 211–233 | KP749877 | 6 | VIC | 0.2 | 0.1 | 0.3 | 57 | 47 | 23 |
Ja23 | CAGGCCATCTTGCTGCAGAT | ACAGCCTCTCCATCATGCGT | (GA)9 | 120–134 | KP749880 | VIC | 0.1 | 0.2 | 0.3 | ||||
Ja39 | CGTGCAGTCATCTCAGTCAG | GCTGAGGAGAGCGAGTAATC | (TC)8 | 128–144 | KP749884 | NED | 0.15 | 0.15 | 0.3 | ||||
Ja55 | ACAACAGCAACAACTTCCGT | AGTTGTGATGTGGAGGAGCA | (CAA)6 | 130–138 | KP749887 | PET | 0.15 | 0.15 | 0.3 | ||||
Ja82 | TCCGAATGCACCAAATCTGA | GGCTTGTATTCGAATTTACATGGA | (GAA)6 | 292–295 | KP749895 | FAM | 0.4 | 0 | 0.4 | ||||