Fig. 5From: Automated amplicon design suitable for analysis of DNA variants by melting techniquesAllele separation of an amplicon with primers selected, controlled and simulated by the Variant Melting Profile. Inserted is the melting profile. Alleles were separated by cycling the temperature from 52–49 °C, 20 times during capillary electrophoresis. Signal was recorded by laser-induced fluorescenceBack to article page