TY - JOUR AU - Ogura, Takahiro AU - Tsuchiya, Akihiro AU - Minas, Tom AU - Mizuno, Shuichi PY - 2015 DA - 2015/11/04 TI - Methods of high integrity RNA extraction from cell/agarose construct JO - BMC Research Notes SP - 644 VL - 8 IS - 1 AB - Agarose hydrogels are widely used for three-dimensional cell scaffolding in tissue engineering and cell biology. Recently, molecular profiles have been obtained with extraction of a minimal volume of RNA using fluorescent-tagged quantitative polymerase chain reaction (qPCR), which requires high integrity RNA. However, the agarose interferes considerably with the quantity and quality of the extracted RNA. Moreover, little is known about RNA integrity when the RNA is extracted from cell/agarose construct. Thus, in order to obtain RNA of sufficient integrity, we examined various extraction methods and addressed reproducible methodologies for RNA extraction from cell/agarose constructs using spectrophotometry and microfluidic capillary electrophoresis. SN - 1756-0500 UR - https://doi.org/10.1186/s13104-015-1627-5 DO - 10.1186/s13104-015-1627-5 ID - Ogura2015 ER -