Table 1 Concentration, purity and integrity of extracted RNA from different methods
From: Methods of high integrity RNA extraction from cell/agarose construct
Concentration (ng/µl) | Purity | Integrity | |||
|---|---|---|---|---|---|
Spectrophotometer | Microfluidic capillary electrophoresis | OD 260/280 | OD 260/230 | RIN | |
TZ (control) | 263.47 ± 49.2 | 67.33 ± 21.39 | 1.93 ± 0.06 | 0.56 ± 0.12 | 8.8 ± 1.04 |
TZ | 862.49 ± 203.73 | 8.67 ± 4.93 | 1.77 ± 0.05 | 0.32 ± 0.085 | n/d |
TZ-Salt | 167.46 ± 26.49 | 8.67 ± 4.93 | 1.86 ± 0.21 | 0.54 ± 0.34 | n/d |
TZ-NTC | 26.10 ± 9.03 | 24.33 ± 7.51 | 2.04 ± 0.17 | 0.85 ± 0.46 | 7.3 ± 0.35 |
TZ-2NTC | 25.04 ± 11.98 | 33 ± 12.17 | 2.02 ± 0.21 | 0.66 ± 0.13 | 7.6 ± 0.15 |
TZ-Salt-NTC | 4.55 ± 2.00 | 5 ± 2.65 | 2.15 ± 0.51 | 0.123 ± 0.032 | n/d |
TZ-Salt-2NTC | 2.45 ± 2.51 | 7 ± 2 | 1.15 ± 1.48 | 0.09 ± 0.12 | n/d |
TZ-Plant | 55.96 ± 33.54 | 34.33 ± 2.31 | 1.84 ± 0.07 | 0.91 ± 0.53 | 8.1 ± 0.15 |
TZ-Direct | 13.05 ± 5.71 | 10.56 ± 6.94 | 1.53 ± 0.14 | 0.71 ± 0.15 | n/d |