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Table 2 Ranking of reference genes based on GeNorm and NormFinder calculations, averages of all evaluated brain areas

From: Reference genes for reverse transcription quantitative PCR in canine brain tissue

Ranking order

GeNorm

NormFinder

NormFinder stability value

1 most stable

HMBS

HMBS

0.005

2

GAPDH

GAPDH

0.007

3

HPRT

HPRT

4

RPL13

RPS19

5

SDHA

GUSB

6

RPS5

RPS5

0.008

7

RPS19

B2M

8

YWHAZ

RPL13

0.009

9

B2M

SDHA

10 least stable

GUSB

YWHAZ

0.025

  1. A low stability value in NormFinder calculations corresponds with high expression stability. The GeNorm program doesn’t distinguish between the two most stably expressed genes (HMBS and HRPT) because gene ratios are required for the calculations