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Table 2 Ranking of reference genes based on GeNorm and NormFinder calculations, averages of all evaluated brain areas

From: Reference genes for reverse transcription quantitative PCR in canine brain tissue

Ranking order GeNorm NormFinder NormFinder stability value
1 most stable HMBS HMBS 0.005
2 GAPDH GAPDH 0.007
3 HPRT HPRT
4 RPL13 RPS19
5 SDHA GUSB
6 RPS5 RPS5 0.008
7 RPS19 B2M
8 YWHAZ RPL13 0.009
9 B2M SDHA
10 least stable GUSB YWHAZ 0.025
  1. A low stability value in NormFinder calculations corresponds with high expression stability. The GeNorm program doesn’t distinguish between the two most stably expressed genes (HMBS and HRPT) because gene ratios are required for the calculations