Fig. 1
From: Effects of oxygen on the antigenic landscape of prostate cancer cells
Effect of hypoxia on PCa cells. Effects on cell viability: VCaP (a), LNCaP (b) and C4-2B (c) cells were cultured at pO2 = 1 or 2 kPa (filled circle) or 20 kPa (circle). Alive cells were counted in triplicate flasks using trypan blue exclusion to differentiate dead cells. Effects on VEGF production: Rates of VEGF release in conditioned culture media were measured by ELISA for VCaP (d), LNCaP (e) and C4-2B (f) cells. Assessment of VEGF transcripts: mRNA levels of VEGF were analyzed in VCaP (g), LNCaP (h) and C4-2B (i) cells by qRT-PCR. *p < 0.05 when data at hypoxia is compared to values at normoxia O2 at the same time point; # p < 0.05 when data at hypoxia or normoxia is compared to a previous time point within the same pO2; & p < 0.05 when a specific data point is compared to the point at day 0 within the same pO2