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Fig. 3 | BMC Research Notes

Fig. 3

From: Effects of oxygen on the antigenic landscape of prostate cancer cells

Fig. 3

Identification of PCa-associated antigens in hypoxic LNCaP cell lysates and patient plasma immune reactivity. LNCaP cells were cultured at pO2 = 2 kPa and 20 kPa conditions for 4 days. Cell lysates were prepared and 50 μg proteins were loaded on pH 3–10 NL IPG strips for 2DGE. One set of gels was silver stained and other set was transferred to nitrocellulose membranes (a), incubated with pooled plasma from patients with PCa, colon cancer, lung cancer, rheumatoid arthritis (an autoimmune disease), and age matched healthy controls. Following incubation with chicken anti-human IgG-HRP, spots were identified by chemiluminescence (b). The arrowheads indicate protein spots of interest

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