Fig. 2
From: Development of repeatable arrays of proteins using immobilized DNA microplate (RAPID-M) technology
PCR amplification of gfp and ompA genes using T7Prom and T7Term primers. Primers for gfp amplification are as follows: lane 1, soluble T7Prom and 5′-T7Term-NH2 (as positive control); lane 2, soluble T7Prom and 5′-T7Term-Bt (as positive control); lane 3, soluble T7Prom and 5′-T7Term-NH2 with gfp-CA beads as template; lane 4, soluble T7Prom and 5′-T7Term-bt with gfp-SA beads as template; lane 5, non-template control (as negative control). Primers for ompA amplification as follows: lane 6, soluble T7Prom and 5′-T7Term-NH2 (as positive control); lane 7, soluble T7Prom and 5′-T7Term-Bt (as positive control); lane 8, soluble T7Prom and 5′-T7Term-NH2 with ompA-CA beads as template; lane 9, soluble T7Prom and 5′-T7Term-bt with ompA-SA beads as template; lane 10, non-template control (as negative control)