Fig. 5
From: Development of repeatable arrays of proteins using immobilized DNA microplate (RAPID-M) technology
Application of the RAPID-M method for generation of protein arrays. The proteins were printed using the Nano-Plotter™ NP 2.1. Immobilized proteins were in situ purified on Ni–NTA chip and subjected to detection using peroxidase-conjugated anti-polyHistidine antibody followed by addition of TSA-Cy5. Lane 1–4, proteins from repetitive cycles of first, second, third and fourth rounds of cell-free expression using immobilized DNA template. Proteins are printed in 3 × 3 blocks and each block represents one cycle of cell-free expression using beads-immobilized DNA