Identification and characterization of microsatellite loci in two socially complex old world tropical babblers (Family Timaliidae)

Table 1 Characteristics of eight microsatellite loci developed and optimized from the chestnut-crested yuhina, Staphida everetti

Locus	Repeat motif	Primer sequence (5′–3′)	T_A (°C)	Size range (bp)	K	H _o	H _E	HWE	GenBank accession no.
StEv26	(CA)₁₃	F: AGCAATAGGACTGACACAAGGT	61–57	84–96	7	0.90	0.70	0.43	KT582127
		R: AGTCTTGATTTCCCCACTTTGTC
StEv103*	(TAA)₁₅	F: CCAGCCTCTGAACTGGTCTG	61–57	228–270	11	0.63	0.66	0.21	KT582120
		R: TTCTGTGGGTCTTGGTGGTT
StEv106	(ATAG)₁₀	F: TTGGACAAACAGCTGCTCCA	61–57	149–201	13	0.80	0.70	0.38	KT582121
		R: ACACACTTCAGCTGGAACTG
StEv110*	(AAAT)₉	F: TCCAGCATTTCTTCCTCTTGGA	62–58	181–199	9	0.58	0.66	0.11	KT582122
		R: AACAGATCCACACAGGCAGG
StEv112	(TTGG)₁₁	F: AGAAGCAGAGAGAGGTAGGAA	61–57	234–258	11	0.89	0.72	0.11	KT582123
		R: TGAGAAAGGATGCACGTGTTG
StEv114*	(AT)₈	F: TCCTTTTCTTTTCCTACTTTCATTTCT	61–57	164–180	6	1.00	0.64	<0.001	KT582124
		R: ACACAGCTTTGTGAGGGCTT
StEv118	(GT)₁₃	F: CACTGCCCAGTTTGGAATGC	61–57	120–152	11	0.84	0.81	0.35	KT582125
		R: CCAGCTCACACTCCTATGCC
StEv122	(AC)₁₀	F: AGTCCTCACTGTGCAGGTTG	61–57	244–252	5	0.74	0.64	0.13	KT582126
		R: ACATGCACACTGTGGACCAA

T _A optimized touchdown upper and lower annealing temperatures, K number of alleles, H _O, observed heterozygosity, H _E expected heterozygosity, HWE test for Hardy–Weinberg equilibrium
* Imperfect microsatellites

ISSN: 1756-0500