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Fig. 6 | BMC Research Notes

Fig. 6

From: Urine is a novel source of autologous mesenchymal stem cells for patients with epidermolysis bullosa

Fig. 6

Isolation and differentiation of USCs from patients with EB. a Representative colonies of USCs isolated from patients with EB are shown. b Chondrogenic differentiation: representative pictures showing morphological changes and alcian blue positive staining of USCs after 18 days of induction, compared to an un-induced control, are depicted (left). The chondrogenic marker Aggrecan could only be detected in induced cultures by semi-quantitive PCR. GAPDH was used as loading control. I induced, C control (right). c Osteogenic differentiation: after 15 days of culture in the induction medium, EB-USCs stained positive for NBT/BCIP in contrast to the un-induced control. Representative images are shown (left). mRNA expression of Osteocalcin (Osteoc.) was analyzed by semi-quantitive PCR. Induced cells showed a significant increase compared to the un-induced control. GAPDH was used as loading control. C control, I induced (right)

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