Fig. 6
From: Urine is a novel source of autologous mesenchymal stem cells for patients with epidermolysis bullosa
Isolation and differentiation of USCs from patients with EB. a Representative colonies of USCs isolated from patients with EB are shown. b Chondrogenic differentiation: representative pictures showing morphological changes and alcian blue positive staining of USCs after 18Â days of induction, compared to an un-induced control, are depicted (left). The chondrogenic marker Aggrecan could only be detected in induced cultures by semi-quantitive PCR. GAPDH was used as loading control. I induced, C control (right). c Osteogenic differentiation: after 15Â days of culture in the induction medium, EB-USCs stained positive for NBT/BCIP in contrast to the un-induced control. Representative images are shown (left). mRNA expression of Osteocalcin (Osteoc.) was analyzed by semi-quantitive PCR. Induced cells showed a significant increase compared to the un-induced control. GAPDH was used as loading control. C control, I induced (right)