A formalin-free method for stabilizing cells for nucleic acid amplification, hybridization and next-generation sequencing

Table 1 HER-2/CEP17 ratio in control and fixed SKBR-3 cells as determined by ddPCR (mean ± SD)

Sample Type	HER-2	CEP17	HER-2/CEP17
Sample Type	Copies/10⁶ cells	Copies/10⁶ cells	Ratio
Frozen control cells	10,288,417 ± 4,535,976	1,465,957 ± 763,934	7.0 ± 1.1
10 % NBF fixed cells	163,256 ± 147,676	3,687 ± 3,815	44.3 ± 12.8
SCP fixed cells	7,380,677 ± 4,069,048	1,063,169 ± 678,786	6.9 ± 1.4

ISSN: 1756-0500