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Table 1 Summary of results from RNA extraction performed with the indicated methods

From: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

Method/kit Total RNA yield (µg) RIN value Purity (A260/A280)
Glucose PE Average
TRI reagent (MRC, USA) 2.83 ± 0.95 2.63 ± 0.25 2.72 ± 0.58 NA 1.94 ± 0.01
RNeasy Mini kit (Qiagen, USA) 6.80 ± 0.20 2.15 ± 0.10 4.48 ± 2.69 9.65 ± 0.24 1.98 ± 0.05
Phenol-free total RNA purification kit (Amresco, USA) 26.55 ± 2.22 14.72 ± 2.23 20.63 ± 7.07 9.85 ± 0.20 2.01 ± 0.03
  1. Total yield indicated from starting cell density of 109 bacterial cells. NA-not available