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Table 2 Characterization of species-specific primers developed for Hyacinth macaw

From: Development of microsatellite markers for Hyacinth macaw (Anodorhynchus hyacinthinus) and their cross-amplification in other parrot species

Locus

Primers sequences (5′–3′)

Repeat motif

Size range

T °C

N

n

Ho

He

Fis

PIC

AnH6

F AAAGGCAGTTCAGGTGTTGG

R ACACACACGCACATACTCCA

(GT)n(AT)n(GT)n

234–236

60–55 °C (td)

44

2

0.500

0.502

0.003

0.373

AnH10

F CCTATACCCAGCTCCCAACA

R AGCCTTCAGTGGCTCATTGT

(AC)×9

166–172

57 °C

57

3

0.175

0.193

0.092

0.179

AnH17

F TTCCCATTGGATATCTTGTCAG

R ATTGGCAATGGCCTAAACAC

(CA)×16

184–192

59 °C

49

5

0.531

0.669

0.217

0.605

AnH23

F TGTGGCATCTGTAAAGAAAGAGG

R GCCTGGGGAGTGATTGTTTA

(AC)×9

222

57 °C

18

1a

AnH33

F GCCTGTGCCAGATGGTAAAT

R GCCCTAAAAATGCTTTCCAA

(TG)×12

177–179

60–55 °C (td)

51

2

0.275

0.363

0.237

0.295

AnH34

F GACAGACACATCCGCTTCAA

R AACACACATCTTCATATGCAACC

(TG)×24

172–210

60 °C

43

12

0.721

0.818

0.119

0.786

    

Mean

 

4.8

0.440

0.509

0.134

0.448

  1. Microsatellite loci, sequence of primers, repeat motifs, fragment size, annealing temperature (T °C), number of used samples (N), number of alleles (n), observed heterozygosity (Ho), expected heterozygosity (He), inbreeding coefficient (Fis) and PIC (polymorphic information content)
  2. td touchdown decreasing 0.5 °C per cycle
  3. aMonomorphic loci