Table 1 Global characterization of the 22 microsatellite loci characterized in Acanthodactylus aureus, A. dumerili/A. senegalensis and A. longipes
Locus | GenBank assess no. | Repeat | Primer sequence (5′–3′) | Multiplex | TD | Dye |
|---|---|---|---|---|---|---|
Ac1 | KU295182 | (ATAC)8 | F: CTGTGGTATATCCCCTGCCA R: GGTGGCTTCTCCACAGCTATT | 1 | 58°/54° | FAM |
Ac4 | KU295183 | (TTC)21 | F: ACAGCTCTGCAGTAATTCCATTT R: CCGATGCAGTGTTTCGTAGG | 3 | 58°/54° | VIC |
Ac5 | KU295184 | (AAC)15 | F: GTTGCTTCAACTGCTCCTCC R: AGTGTCCTGTGCACAACCAG | 1 | 58°/54º | VIC |
Ac6 | KU295185 | (TTG)10 | F: GTAGCCCAGTCAGATGGGTG R: CCTCCAACATTCCAGTCCAG | 4 | 55°/51° | NED |
Ac8 | KU295186 | (TTG)11 | F: GACATCTGAAGGCAGCCCTA R: GGTTGTAGCCTGGAGCAGAA | 1 | 58°/54° | NED |
Ac9 | KU295187 | (CAA)15 | F: TCATACAGGGATGTTTCAGGG R: GCAGGAGGAAGGAAGCTTTT | 1 | 58°/54° | PET |
Ac13 | KU295188 | (AAC)14 | F: TCCATGGGGTCACAAAGAGT R: TCTCCAGCACTTATCTGATGC | 2 | 58°/54° | FAM |
Ac14 | KU295189 | (CAA)10 | F: TTAAGTGGCAATGTGTTGCAT R: TCCCACATGGTGGGTTACTT | 2 | 58°/54° | VIC |
Ac16 | KU295190 | (AGG)10 | F: AGTCAATTTATTCAAATGATCTTCCA R: TCATCCAAGAAAATCTGCTGC | 2 | 58°/54° | VIC |
Ac19 | KU295191 | (AAC)14 | F: TCATTTCACTTCAAACCTGTGG R: ACTGATGTTGGGTTTGGAGC | 2 | 58°/54° | PET |
Ac20 | KU295192 | (GTT)11 | F: ATGCATAAGTACGAAAAGGGGA R: TCTACAGAGAAAGAGAAATAACAACAA | 2 | 58°/54° | PET |
Ac23 | KU295193 | (CAT)8 | F: GCGAACATGCACAAGGTTT R: ACCCTGCTTGGTTCTCATTG | 1 | 58°/54° | FAM |
Ac28 | KU295194 | (ACAT)8 | F: TGTCCGAAATAGGATGGAGC R: GGAAAGCCAATGCCTCTACA | 4 | 55°/51° | PET |
Ac31 | KU295195 | (GTT)10 | F: GAAGGGTTACAACTGCCTGG R: CAGTGCTTCAGCAACAGGAG | 4 | 55°/51° | FAM |
Ac32 | KU295196 | (TTC)15 | F: TAGTCCGTAAACTTGTGGGTCA R: TTCTCAGACAACAGACACCCA | 3 | 58°/54° | FAM |
Ac33 | KU295197 | (TGT)16 | F: GGCACTGAAATATGTGGTTTTG R: TGACATGCTTCGGTGAAGTC | 3 | 58°/54° | FAM |
Ac36 | KU295198 | (TGT)9 | F: GTCACGTTGATTGCATTGCT R: GCCAACTGGGAAACCTAGC | 3 | 58°/54° | VIC |
Ac43 | KU295199 | (CAA)13 | F: AGCTTTTGTACGTTCCTTTGC R: CCAGAGAAACACATATGCAAGC | 4 | 55°/51° | FAM |
Ac44 | KU295200 | (GGA)11 | F: TCCTTAAGAAAGGTACTTAATGCCA R: TCTTTACGTAGTCCCTTTGTGG | 4 | 55°/51° | VIC |
Ac45 | KU295201 | (CAA)10 | F: AGGCAATGGAAGACAGGGA R: GCCTACAGTTTGTGCATAGGG | 4 | 55°/51° | VIC |
Ac47 | KU295202 | (ACA)11 | F: CTTGCCTCTTCGCTTTCTGT R: TCCGGACAGCATTCCTCTAC | 4 | 55°/51° | NED |
Ac49 | KU295203 | (AAC)11 | F: CAAAGAAAATTGTTGGAGGGG R: GTAAAACATCGGAAGGCAGC | 4 | 55°/51° | PET |