Fig. 2

Viral protein expression and electron microphotographs. a The expressions of EV71 structure proteins were visualized by SDS-PAGE. b The expression of EV71 VP2 was monitored using VP2-specific antibody MAB979 by Western blot. EV71 VLPs were isolated using sucrose and discontinuous cesium chloride (CsCl) gradient (1.4, 1.33, 1.29 and 1.25 g/ml) by ultracentrifugation. The formation of EV71 VLPs in yeast was monitored by electronic microscope. Size bar 50 nm