A cancer cell-line titration series for evaluating somatic classification

BMC Research Notes

Table 1 Sequencing quality control metrics

Library	Read length	Error % (%)	Soft clip % (%)	% Reads on target (%)	# Reads on target	Average coverage	90 % covered at
100 % Cell-line	2 × 101	0.18	1.76	65.34	164,598,000	326.04×	>22×
60 % Cell-line	2 × 101	0.19	1.54	67.30	154,878,000	307.48×	>21×
40 % Cell-line	2 × 101	0.18	1.59	66.29	171,334,000	339.97×	>24×
20 % Cell-line	2 × 101	0.29	1.18	67.25	153,932,000	306.70×	>21×
15 % Cell-line	2 × 101	0.18	1.52	66.05	182,028,000	361.46×	>25×
10 % Cell-line	2 × 101	0.20	2.13	66.03	184,641,000	364.35×	>27×
5 % Cell-line	2 × 101	0.21	1.70	67.40	186,666,000	369.99×	>29×
100 % Normal	2 × 101	0.23	1.78	66.81	200,737,000	397.55×	>27×

Several quality control metrics are reported here for the cell-line titration series libraries, as determined from Novoalign mapped reads. Error % is defined as the number of bases mapped as mismatches or indels over the total number of mapped bases. An on target read is defined as any read with at least one base overlapping the Agilent SureSelect All Exon v4 target regions

ISSN: 1756-0500