Fig. 1

Procedural overview for the isolation of RNA from fresh frozen microdissected prostate tissue. Primary prostate tissue was sectioned and the sections were transferred to a membrane slide. After staining, areas of interest were microscopically identified and excised by microdissection. RNA was extracted from the tissue fragments using one of several commercial kits. Quality and quantity of RNA were measured with the Agilent Bioanalyzer. In a selection of RNA samples, the amount of two transcripts characteristic of prostate cancer were measured by quantitative PCR