Fig. 1

LL-37 reduces RSV-A2 infection of and replication in ALI Calu-3. ALI—cultured Calu-3 cells were infected at the apical surface with RSV-A2 at MOI = 1, or treated prophylactically or therapeutically with 50 μg/ml LL-37 or sLL-37. Prophylactic treatment was defined as a 1 h co-incubation of virus with peptide immediately prior to infection. Therapeutic treatment was defined as inclusion of peptide in the basolateral medium, beginning 24 h pi, with replacement of medium and peptide at 3 days pi. Total cellular RNA was isolated from five replicates per infection condition at 7 days pi, and the relative level of RSV-M gene was determined by qRT-PCR. Data is presented as the mean relative level of RSV-M gene, in genome equivalents/ml, of five replicates ± SD. Asterisk indicates p ≤ 0.05 as determined by unpaired two-tailed analysis. The data presented in this figure is representative of three independent experiments