Fig. 2
From: Molybdate transporter ModABC is important for Pseudomonas aeruginosa chronic lung infection
STM_modA is unable to perform anaerobic growth and use nitrates without molybdate supplementation. a anaerobic growth curves in LB medium supplemented with 15 mM KNO3, plus 100 μM sodium molybdate when indicated. STM_modA is unable to grow in anaerobic conditions with nitrates as the terminal electron acceptor without molybdate supplementation. Results are from three independent experiments; error bars represent standard deviation b anaerobic overnight growth in LB medium supplemented with 15 mM KNO3, plus 100 μM sodium molybdate when indicated. Addition of molybdate restored growth in the STM_modA mutant. The complemented STM_modA strain showed growth without molybdate supplementation. Statistical significance was assessed using an ANOVA in GraphPad prism 6.0 (*** p = 0.0003; **** p < 0.0001). c Residual nitrate dosage in the medium after overnight growth using the Griess reaction. Nitrates levels are inversely proportional to nitrate uptake and utilization. Adding molybdate to the medium restored the STM_modA mutant’s nitrate consumption. Results for b and c are for three technical replicates in one of three consistent experiments