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Table 1 Strains and plasmids

From: Molybdate transporter ModABC is important for Pseudomonas aeruginosa chronic lung infection

Strain or plasmid Relevant characteristics Source or reference
E. coli strain
NEB 5α Chemically competent cells New England Biolabs
P. aeruginosa strains
PAO1 PAO1293, CmS, wild-type, derivative of PAO2 which originates from PAO1 [48]
STM_modA PAO1 STM_modA::miniTn5-Km2 mutant inactivating modA (PA1863), KmR [15]
Complemented STM_modA PAO1 STM_modA with pUCP19::modABC plasmid (PA1863, PA1862, PA1861) This study
Plasmids
pUCP19 CbR, cloning vector and used for CI [49]
pUCP19 ::modABC CbR, KmR, modABC genes on pUCP19 vector This study