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Fig. 2 | BMC Research Notes

Fig. 2

From: Optimization of canine interleukin-12 production using a baculovirus insect cell expression system

Fig. 2

Optimization of canine IL-12 single-chain protein expression in the baculovírus-insect cell system. High-Five cells were infected with distinctive baculovírus constructs using different multiplicity of infection (MOI) and time of infection (TOI). Dot-blot assay was carried out using 150 μL of infected cell culture supernatant proteins impregnated on nitrocellulose membrane and anti-His C-Term antibodies followed by anti-mouse immunoglobulin-alkaline phosphatase conjugate and substrate

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