Determining the order of resistance genes against Stagonospora nodorum blotch, Fusarium head blight and stem rust on wheat chromosome arm 3BS

Table 1 Summary information of the three wheat cultivars contributing genes for resistance against Stagonospora nodorum blotch, Fusarium head blight and stem rust and summary of simple-sequence repeat markers linked to each resistance gene used for genotyping parental wheat lines during the initial analysis of 400 F₂ progeny (Xgwm389) and for all progeny sets (Xumn10)

Cultivar	Growth habit	Resistance gene	Genetic control	Linked SSR markers^a	Fragment sizes	Reference
Arina	Winter	QSng.sfr-3BS	Polygenic	Xgwm389	134-135	[37]
Alsen	Spring	Fhb1 or QFhs.ndsu-3BS	Additive	Xumn10	241-242	[16]
Ocoroni86	Spring	Sr2	Recessive	Xgwm389	115-119	[47]

^aThe same PCR conditions were utilized for all SSR markers: initial denaturation at 95 °C for 4 min; 30 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s; 30 cycles of 95 °C for 30 s, 45 °C for 30 s, and 72 °C for 30 s; followed by a final elongation at 72 °C for 7 min and a hold at 4 °C

ISSN: 1756-0500