Determining the order of resistance genes against Stagonospora nodorum blotch, Fusarium head blight and stem rust on wheat chromosome arm 3BS

Table 2 List of Kompetitive Allele-Specific PCR (KASPar) single-nucleotide polymorphism (SNP) markers and their sequences used to genotype 1600 F₂ progeny derived from a cross of Arina (Qsng-sfr-3BS) by two F₂ progeny from a cross between Alsen and Ocoroni86 that were homozygous for the molecular markers linked to the Fhb1 (Alsen) and Sr2 (Ocoroni86) resistance genes

Primer name^a	Sequence	Fluorescent label
Xsnp3BS-2_ALA	GAAGGTGACCAAGTTCATGCTGGTCTCGGAATTGATTGTAGAAGGTA	FAM
Xsnp3BS-2_ALC	GAAGGTCGGAGTCAACGGATTGTCTCGGAATTGATTGTAGAAGGTC	VIC
Xsnp3BS-2_C1	CCAGAGACTACATAKCCTTTTCTTACAAT
Xsnp3BS-3_ALG	GAAGGTGACCAAGTTCATGCTTTCAAGTGCTGGCATTGCATCC	FAM
Xsnp3BS-3_ALA	GAAGGTCGGAGTCAACGGATTCTTTCAAGTGCTGGCATTGCATCT	VIC
Xsnp3BS-3_C1	GCTTCTTYGCACCTCTAGTGCCAA
Xsnp3BS-9_ALC	GAAGGTGACCAAGTTCATGCTTTCAAGAAAGCTTCTGCCAGTCAC	FAM
Xsnp3BS-9_ALG	GAAGGTCGGAGTCAACGGATTTCAAGAAAGCTTCTGCCAGTCAG	VIC
Xsnp3BS-9_C1	AAAAGAACAGACACGCCAGGATTCAAAA
g01130_AL1	GAAGGTGACCAAGTTCATGCTATAGCTYCATCTCATTTCCTCCTTG	FAM
g01130_AL2	GAAGGTCGGAGTCAACGGATTCATAGCTYCATCTCATTTCCTCCTTA	VIC
g01130_C1	TAGAACCAAAGCKTCAAACATTTCTGTGAA

^aPrimer name indicates the published markers from which the sequences were obtained for design of the KASP assay, with the addition of the allele-specific designation. The common primer is indicated as C1

ISSN: 1756-0500