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Table 1 Comparison of the performance of the four algorithms implemented in OPENDoRM

From: A web-oriented software for the optimization of pooled experiments in NGS for detection of rare mutations

  NoReplica OptReplica Transposition DiagWalks
Num Patients 5 5 6 6
Num pools 13 22 22 23
Num Sanger test 1580 0 0 20
Expected patient 64 64 56 25
Total cost (€) 2640 22,000 22,000 23,160
Num patients 4 5 6 6
Num pools 32 22 22 23
Num Sanger test 3584 0 0 20
Expected patient 128 128 113 49
Num patients 3 20 20 20
Num pools 667 200 200 200
Num Sanger test 119,960 0 0 160
Expected patient 2000 2000 1901 361
Total cost (€) 1,626,680 200,000 200,000 201,280
  1. Case study 1: The input parameters are: number of patients = 64; max Poolsize = 6; number of mutations to detect = 5; cost of a single Sanger test = €8; cost of a single pool for NGS = €1000. Case study 2: The input parameters are: number of patients = 128; max Poolsize = 8; number of mutations to detect = 7; cost of a single Sanger test = €10; cost of a single pool for NGS = €1000. Case study 3: The input parameters are: number of patients = 2000; max Poolsize = 20; number of mutations to detect = 20; cost of a single Sanger test = €8; cost of a single pool for NGS = €1000