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Fig. 2 | BMC Research Notes

Fig. 2

From: Development of safe, effective and immunogenic vaccine candidate for diarrheagenic Escherichia coli main pathotypes in a mouse model

Fig. 2

Evaluation of the effect of adjuvant system on survival rate in mice immunized with combined or individual antigens. Balb/C mice were double immunized subcutaneously with 109 CFU of formalin killed whole cell antigens. Antigens belonged to the above-mentioned five-diarrheagenic E. coli pathotypes. Combined vaccine candidate consisted of formalin-killed whole cells of the main five pathotypes. Two weeks later, mice were challenged intraperitoneally with 106 CFU of respective living E. colipathotype. Evaluation of the effect of adjuvant system on the survival rate of mice groups post immunization with a combined vaccine candidate, b EAEC antigens, c EPEC antigens, d EIEC antigens, e EHEC antigens, and f EHEC antigens. In each group, appropriate controls were used. p < 0.05 were considered statistically significant when comparing immunized groups to formalin and PBS controls

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