Fig. 8
Evaluation of IFNγ levels elicited by combined vaccine candidate. Balb/C mice (n = 10 mice per group) were immunized subcutaneously with 109 CFU of formalin killed whole cell antigens. Antigens belonged to the above-mentioned five-diarrheagenic E. coli pathotypes. Combined vaccine candidate consisted of formalin-killed whole cell of the main five pathotypes. Post-immunization blood samples were collected from mice groups weekly for 6 weeks. At week seven, mice were challenged with 106 CFU intraperitoneally and blood samples were collected 1 week after the challenge. The concentration of IFNγ was measured for all seven intervals. IFNγ concentration of CTB-adjuvanted combined vaccine candidate at selected time points compared to a EAEC antigens, b EPEC antigens, c EIEC antigens, d EHEC antigens and e ETEC antigens. *p < 0.05, ** p < 0.001, and ***p < 0.0001, each bar represents mean ± standard deviation