Fig. 2From: An optimized staining technique for the detection of Gram positive and Gram negative bacteria within tissueIn vitro validation of applied stains. Routine Gram stain shows identification of Gram negative (a P. aeruginosa) and Gram positive (b S. aureus) bacteria in both large aggregates (dotted lines) and individual bacteria within the biofilm. Selected P. aeruginosa (c) and S. aureus (d)-antibodies were used to confirm species identity of cultures used for ex vivo studiesBack to article page