Fig. 1

The evolution of the reconstruction of a genic sequence, represented by a succession of exons (orange) and intron (green). The paired-end reads are colored in orange or in green if they correspond to an intronic sequence (green), an exonic sequence (orange) or a junction intron/exon sequence (green and orange). The figure shows that during the iterations, the introns are added to the exons present in the first reference sequence (Unigene), providing additional information for the next iteration